E3451-49A
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Bo Ca Hu Mo RtAccession #
NP_829883.1, NP_829884.1Gene ID
23085, 111173Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-ERC1 (ELKS/Rab6-interacting/CAST Family Member 1, ERC-1, Rab6-interacting Protein 2, ELKS, KIAA1081, RAB6IP2)
Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 0.1-0.3ug/ml, observed in human pancreas lysates on ~90kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to YGSARSVGKVEPS-C, from human ERC1, at N-terminal (NP_829883.1, NP_829884.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ERC1. Species sequence homology: Bovine, canine, mouse and rat.
