Technical Data

E3455-10R
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC N WB
Crossreactivity
Hu
Accession #
NP_000790.2
Gene ID
2056
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Erythropoietin (EPO, EPO alpha, EP, MGC138142, MVCD2)
Erythropoietin Precursor, Epoetin

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological levels of circulating erythrocyte mass. It is often used as a drug to treat anemia.

Applications
Suitable for use in ELISA, Functional Assays, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): 15ug/ml ELISA: 0.5-1ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human Erythropoietin expressed in CHO cells.
Form
Supplied as a liquid in PBS, pH 7.2, <5% trehalose.
Purity
Purified by Protein A affinity chromatography. |Endotoxin: <0.1EU/ug (LAL)
Specificity
Recognizes human Erythropoietin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Krystal, G., et al. (1983) A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice. Exp. Hematol. 11: 649-660. 2. Kessler, S.W., et al. (1975) Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol 115: 1617-1624.
USBio References
No references available
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