Technical Data

E3500-37L-APC
Clone Type
Monoclonal
Host
Mouse
Source
E. coli
Conjugate
APC
Isotype
IgG2b
Clone Number
10J22
Grade
Affinity Purified
Applications
FLISA IF
Crossreactivity
Ec
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
BSA Free
Mouse Anti-Escherichia coli, O157 (E. coli) (APC)
Applications
Suitable for use in Immunofluorescence and FLISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partially purified E. coli O157 LPS
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography from ascites
Specificity
Recognize the “O” antigen of E. coli serotype O157. Crossreactivities: Salmonella O30 (Hilversum) and Salmonella O30 (urbana). Non-reactive with E. coli serotypes O111, O125, O20 and O55

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net