E3550-01G-ML550
Clone Type
MonoclonalHost
MouseConjugate
MaxLight™550Isotype
IgG1Clone Number
4S24 (BGN/06/8824)Grade
Affinity PurifiedApplications
FLISAShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Estradiol (17b-Estradiol; 17beta-Estradiol) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilution
FLISA: 1:5,000-1:50,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
17beta estradiol conjugated to BSA at position 6.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes Estradiol 17b. Reactivites observed: 17beta Estradiol 100%, 17beta EstradiolBSA 90%, Estriol <0.9%, Testosterone <0.01%.
