Technical Data

E3564-82
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
6F11
Grade
Supernatant
Applications
IHC IP WB
Crossreactivity
Hu Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Estrogen Receptor (ER)

The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yaziji et al. 2008). ~75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy (Bhargava et al. 2012).

Applications
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Frozen): 1:40-1:60, the use of Zamboni's fixative is recommended Immunohistochemistry (Paraffin): 1:40-1:80, requires antigen retrieval using heat treatment prior to staining of paraffin sections. It is recommended to use sodium citrate buffer pH 6.0 for this purpose. Western Blot: 1:50-1:100 for 2 hrs. at RT Immunoprecipitation (denatured verified): 10ul/mg protein lysate. Use Protein G. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to full length human estrogen receptor.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Tissue culture supernatant
Specificity
Recognizes human Estrogen Receptor at 67kD. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Green S; Chambon P. (1986) Nature 324(6098):615-7. 2. Green S; Gronemeyer H; et al. (1987) Chichester, England, Ellis Horwood Ltd. 7-28. 3. Evans RM, (1988) Science 240:889-95. 4. Danielson M; Northrop JP; et al. (1986) EMBO Journal 5:2513-22. 5. Kumar V, et al., (1987) Cell 51(6):941-51.|6. Greene GL; Sobel BN; et al., (1988) Molecular Endocrinology 2:714-26. 7. Jensen EV; Jacobson HI. (1962) Recent Progress in Hormone Research 18:387-414. 8. Walter P, et al., (1985) Proc Nat Academy of Sciences of the United States of America 82(23):7889-93. 9. Green S, et al., (1986) Journal of Steroid Biochemistry 24(1):77-83. 10. Greene GL, et al., (1986) Science 231(4742):1150-4.
USBio References
No references available
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