Rabbit Anti-Estrogen Receptor alpha, phosphorylated (Ser104) (ER, ER alpha, Era, ESR, ESR, ESR1, ESRA, Estradiol Receptor, Estrogen Receptor, Estrogen Receptor 1)
Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha. Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Estrogen Receptor alpha phosphorylated at Ser104.
Form
Supplied as a liquid.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ERa when phosphorylated at serine 104. Species Cross-Reactivity: mouse.
