Technical Data

E3565-17-APC
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
APC
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
BSA Free
Rabbit Anti-Estrogen Receptor, beta (ERb) (APC)

Estrogen receptor (ER) is a member of the steroid-receptor family. Unlike protein growth factors that bind to receptors on the cell surface and activate signal-transduction cascades to influence gene expression, the steroid hormones bind to intracellular receptors, which then bind to DNA and regulate gene expression directly. ER-b has a molecular mass of approximately 53kD and is composed of six regions, A to F, on the basis of different amino acid sequence homology (2). The A/B region contains a transcription activation function. Region C encodes the DNA binding domain. Region D is involved in binding to hsp90, as well as containing nuclear localization signals and plays a part in stabilizing DNA binding by the DNA binding domain. Region E contains the hormone/ligand binding domain as well as hormone-inducible transcription activation function. Region F appears to play a role in modulating transcriptional activation by ER-a. ER-b was discovered in 1996 in rat prostate, as well as in secretory epithelial cells of the prostate and granulose cells of the ovary (1). Later, human ER-b was discovered and characterized and found to be expressed in human thymus, spleen, ovary and testis (3).

Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
A 19 amino acid synthetic peptide derived from the C-terminus of the human Estrogen Receptor beta protein.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Estrogen Receptor beta protein (~53kD). Reactivity was confirmed by Western Blot using recombinant human Estrogen Receptor beta and MCF-7 cell lysate.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kuiper, G., et al., (1996) Proc. Natl. Acad. Sci. USA 93(12):5925-5930. 2. Krust, A., et al., (1986) Embo J. 5(5):897-897. 3. Mosselman, S., et al., (1996) FEBS Lett 392(1):49-53.
USBio References
No references available
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