Technical Data

E3565-17Z
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
5/25
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu
Gene #
ESR2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Estrogen Receptor beta5 (ERb5)

Eestrogen receptor beta 5 (ERB5) is a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription. Immunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied (1). Studies also revealed the nuclear expression of ERB5 in the majority of breast carcinomas tested, with a possible association with improved patient survival (2).

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Paraffin): Requires antigen retrieval using sodium citrate buffer pH 6.0 heat treatment Optimal dilutions to be determined by the researcher.
Positive Control
Normal colon or colon carcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Tuberculin conjugated synthetic peptide, LLSHVRHARYAP, corresponding to the C-terminus of human ERB5.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human estrogen receptor beta 5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Wong, N. et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates. J. Pathol. 207: 53-60. 2. Shaaban, A.M. et al. (2008) Nuclear and cytoplasmic expression of ERbeta1, ERbeta2, and ERbeta5 identifies distinct prognostic outcome for breast cancer patients. Clin. Cancer Res. 14: 5228-5235.
USBio References
No references available
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