Rabbit Anti-ETFDH, NT (Electron Transfer Flavoprotein-ubiquinone Oxidoreductase, Mitochondrial, ETF-ubiquinone Oxidoreductase, ETF-QO, Electron-transferring-flavoprotein Dehydrogenase, ETF Dehydrogenase)
Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67-kD precursor which is targeted to mitochondria and processed in a single step to a 64-kD mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-terminal region of human ETFDH (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ETFDH.
