Rabbit Anti-EIF2AK1, ID (Eukaryotic Translation Initiation Factor 2 alpha Kinase 1, Heme-regulated Eukaryotic Initiation Factor eIF-2-alpha Kinase, Heme-regulated Inhibitor, Heme-controlled Repressor, HCR, Hemin-sensitive Initiation Factor-2 alpha Kinase, PRO1362, HRI, KIAA1369)
The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme.
Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 267-296 in the central region of human EIF2AK1.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human EIF2AK1.
