Mouse Anti-Exendin 4
Exendin-4 is a 39-amino-acid peptide found in venom from the Gila monster Helicoderma suspectum. It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to Exendin 4. Epitope: aa9-39
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A/G affinity chromatography from culture supernatant
Specificity
Recognizes Exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells. There is full reaction with exendin-4 (9-39) when used as coat in ELISA showing an epitope in the 9-39 region. Binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between this product and exendin-4 is disrupted by 4.5M MgCl2.
