Mouse Anti-Factor VII (F.VII, Stable Factor, Proconvertin)
Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis. The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor. After cleavage of a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39 to 190) and the heavy chain (residues 191 to 444).
Applications
Suitable for RIA, ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: Coating: 5-10ug/ml; Secondary: 100-1000ug/ml Western Blot (Reduced and oxidized forms): 5ug/ml Optimal dilutions to be determined by the researcher.
Extinction Coefficient
E1%, 1cm, 280nm = 14.0
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human Factor VII
Form
Supplied as a liquid in ddH2O, 50% glycerol
Purity
Purified by gel filtration and ion exchange chromatography.
Specificity
Recognizes human Factor VII, VIIa, BFPRck Factor VIIa. Does not recognize murine or porcine VII via Western Blot on plasma samples.
