F0017-08B
Clone Type
PolyclonalHost
SheepSource
HumanConjugate
HRPIsotype
IgGGrade
Affinity PurifiedApplications
ECrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Sheep Anti-Factor IX (HRP)
Factor IX (F.IX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of F.IX is normally around 5ug/ml (87nM) in plasma. The biological importance of F.IX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity and normal antigen) defect in F.IX function. In its zymogen form F.IX is a single chain molecule of 55,000 daltons. It contains two EGF-like domains and an amino-terminal domain containing 12 gamma-carboxy-glutamic acid (Gla) residues. These Gla residues allow F.IX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146- Ala147 and between Arg180-Val181. The terminal activated product in either case is F.IXab, a two-chain enzyme consisting of a heavy chain (28kD), a light chain (18kD) and an activation peptide product of 11kD. F.IX can also be cleaved into inactive products by thrombin and by elastase. The activity of F.IXab in plasma is inhibited by antithrombin and this inhibition is accelerated approximately 1000-fold in the presence of optimal concentrations of heparin(1-3).
Applications
Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Rz ratio (Reinheitszahl, A403/A280)
0.47 as determined spectrophotometrically.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human Factor IX isolated from plasma.
Form
Supplied as a liquid in buffered stabilizer solution, 50% glycerol. No preservatives added. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Factor IX.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Lawson, JH, Mann KG; Cooperative Activation of Human F.IX by the Human Extrinsic Pathway of Coagulation; J. Biol. Chem. 266, 11317-11327, (1991). 2. Enfield DL, Thompson AR; Cleavage and Activation of Factor IX by Serine Proteases; Blood 64, 821-831, (1984). 3. Limentani SA, Furie BC, Furie B, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 94-108, J.B. Lippincott Co., Philadelphia PA, USA, (1994).USBio References
No references available