Mouse Anti-Factor XI
Human Factor XI is a plasma glycoprotein which circulates in a non-covalent complex with high molecular weight kininogen. The mature molecule is synthesized in the liver. It is a two-chain homodimer with a molecular weight of ~160kD. It is estimated that 5% of the total mass is attributable to carbohydrate. The two identical monomers have molecular weights of 80kD. They are joined together by disulfide bonds. By SDS-PAGE analysis, factor XI appears as a single band both non-reduced (Mr 160kD) and reduced (Mr 80kD).
Applications
Suitable for use as an inhibitor in clotting assays, Western Blot, RIA and ELISA. Useful for purification of Factor XI. Other applications have not been tested.
Recommended Dilutions
Western Blot: Non-reduced samples only Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid, 50% glycerol. No preservative added.
Purity
Purified by ammonium sulfate precipitation, ion exchange chromatography and gel filtratation from ascites.
Specificity
Recognizes human Factor XI.
