Rabbit Anti-FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases (2,3), PI3K and PLCg (4,5). The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the carboxy-terminal region of FAK (6,7).
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (paraffin): 1:800 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid residue 710 of human FAK. Species sequence homology: chicken
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chramotography.
Specificity
Detects endogenous levels of human FAK protein. Does not crossreact with other proteins. Species Crossreactivity: mouse, rat, monkey, bovine and porcine
