Rabbit Anti-FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3)
FAK is a widely expressed non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. Regulation of FAK includes phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phophorylation occurs upon integrin engagement with fibronectin. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells begin to enter the M-phase of the cell cycle. In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK binds multiple signaling proteins including p130Cas, Graf, Grb2, Src family SH2 domains, and the p85 subunit of PI3-kinase. The epitope is localized in the C-terminus (amino acid residues 853-1052).
Applications
Suitable for use in Immunofluorescence, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:10-1:50 Western Blotting: 5.0 _g/mL with an incubation for 2 hours at RT Immunoprecipitation: 10.0 _g/mg of protein lysate
Optimal dilutions to be determined by the researcher.
Recommended Positive Control: HUVEC, NIH3T3 cells, endothelial cells, or kidney tissue.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to amino acid residues 853-1052 from the C-terminal region of human FAK.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes a protein of Mr125kD, identified as focal adhesion kinase (FAK). Species Reactivity: Human, mouse, rat, canine and frog.
