Rabbit Anti-FADS1 (Fatty Acid Desaturase1)
Fatty acid desaturases (FADS) are enzymes that convert a single bond between two carbon atoms (C–C) to a double bond (C=C) in a fatty acyl chain. The resultant double bond is often referred to as an unsaturated bond, and the reactions catalyzed by these enzymes are known as desaturation reactions. These reactions require molecular oxygen and occur under aerobic conditions. The distribution of fatty acid desaturases is almost universal. The enzyme has been found in all organisms examined, with the exception of some bacteria such as Escherichia coli. There are three types of fatty acid desaturase: FADS1, FADS2 and FADS3. FADS1 encodes a deduced 444-amino acid protein that shares a high degree of homology with FADS2 and FADS3. FADS1: Rat: 447 aa; Human: 444 aa; Mouse: 447 aa; ~52kD; Chromosome: 19A. Highly expressed in cerebrum, liver and bone.
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
ELISA: 1:100,000 using 50-100ng control peptide/well Optimal dilutions to be determined by the researcher.
Control Peptide
F0020-04: FADS1 Mouse, Control Peptide (Fatty Acid Desaturase1)
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 17aa near the N-terminus of mouse FADS1, conjugated to KLH.
Form
Supplied as a lyophilized powder, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes mouse FADS1. No significant homology to human and rat FADS1 or to mouse FADS2 and FADS3.
