F0024-43A
Clone Type
PolyclonalHost
GoatSource
RatIsotype
IgGGrade
Affinity PurifiedApplications
E FC IF WBCrossreactivity
Bo Ca Hu Mo Po RtAccession #
NP_001025031.2Gene ID
286151, 78803, 315034Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-FBXO43 (F-box Only Protein 43, Endogenous Meiotic Inhibitor 2, EMI2, FBX43)
Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-PGSAQSKRNLKRL, from rat FBXO43, at C-terminal (NP_001025031.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat FBXO43. Species sequence homology: Bovine, canine, human, mouse and porcine.
