Mouse Anti-Fc Receptor-like 4 (FCRL4, FcRH4)
FCRL4/FcRH4, also known as IRTA1(immunoglobulin superfamily receptor translocation associated1), is a member of the Ig superfamily. It shares sequence homology with the classical Fc receptors. FCRL4 is preferentially expressed in B cells and contains three potential ITIM motifs in its cytoplasmic domain. The gene for FCRL4 is localized to the human chromosome 1q 21-23 region, a hotspot for translocation events.
Applications
Suitable for use Flow Cytometry and Direct ELISA. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a reaction volume not exceeding 200ul. Optimal dilutions to be determined by the researcher.
Hybridoma
Myeloma cells with B cells from Balb/c mice.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Gln16-Asp385 from the extracellular domain of human FCRL4 expressed in NSO cells (NP_112572).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human FCRL4. Does not cross-react with recombinant human FCRL1.
