F3965-06B
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Ca Hu Mo RtAccession #
NP_005094.1Gene ID
9638, 235180, 81730Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-FEZ1 (Fasciculation and Elongation Protein zeta 1, Zygin-1, Zygin I)
This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 0.01-0.03ug/ml, observed in human brain, frontal cortex lysates on ~45kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-QNKQKEQRELMKK, from human FEZ1, at the internal region of the protein (NP_005094.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human FEZ1. Species sequence homology: Canine, mouse and rat.
