F4102-03C
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Goat Anti-Fetuin-A (AHSG)
Fetuin-A (AHSG),59kD glycoprotein, consisting of two cystatin-like domains and a smaller unrelated domain, is predominantly sinthetized in liver . It is secreted into the blood stream and deposited as a noncollagenous protein in mineralized bones and teeth. Fetuin-A occurs in high serum concentrations during foetal life, whereas its level declines following infection, inflamatory and malignancy. Fetuin-A acts as an important circulating inhibitor of ectopic calcification, frequent complication of many degenerative diseases. Low serum level of Fetuin-A is associated with vascular and valvular calcification, atherosclerosis, malnutrition and higher cardiovascular mortality in chronic renal failure, liver cancer and liver cirrhosis patients on long-term dialysis. AHSG protein represents a natural inhibitor of tyrosinase kinase activity of the insulin receptor. It may play a significant role in regulating postprandial glucose disposal, insulin sensitivity, weight gain, and fat accumulation. The serum and bone-resident Fetuin-A binds to transforming growth factor- beta and blocks TGF- beta binding to cell surface receptors.
Applications
Suitable for use in ELISA, Immunoturbidimetry and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stabillty
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Fetuin-A from human serum
Form
Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O. Let the lyophilized pellet dissolve completely.
Purity
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human Fetuin-A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Weikert C, Stefan N, Schulze MB, Pischon T, Berger K, Joost HG, Haring HU, Boeing H, Fritsche A. |Plasma Fetuin-A Levels and the Risk of Myocardial Infarction and Ischemic Stroke. Circulation. |2008 Nov 24; |2. Stefan N, Fritsche A, Weikert C, Boeing H, Joost HG, Haring HU, Schulze MB. Plasma Fetuin-A |Levels and the Risk of Type 2 Diabetes. Diabetes. 2008 Jul 15; |3. Arnaud P, Kalabay L. Alpha2-HS glycoprotein: a protein in search of a function. Diabetes Metab |Res Rev. 2002 Jul-Aug; 18(4): 311-4. Review. |4. Ketteler M, Bongartz P, Westenfeld R, Wildberger JE, Mahnken AH, Bohm R, Metzger T, Wanner C, |Jahnen-Dechent W, Floege J. Association of low fetuin-A (AHSG) concentrations in serum with |cardiovascular mortality in patients on dialysis: a cross-sectional study. Lancet. 2003 Mar 8; |361(9360): 827-33. |5. Jersmann HP, Dransfield I, Hart SP. Fetuin/alpha2-HS glycoprotein enhances phagocytosis of |apoptotic cells and macropinocytosis by human macrophages. Clin Sci (Lond). 2003 Sep; 105(3): |273-8. |6. Schafer C, Heiss A, Schwarz A, Westenfeld R, Ketteler M, Floege J, Muller-Esterl W, Schinke T, |Jahnen-Dechent W. The serum protein alpha 2-Heremans-Schmid glycoprotein/fetuin-A is a |systemically acting inhibitor of ectopic calcification. J Clin Invest. 2003 Aug; 112(3): 357-66. |7. Denecke B, Graber S, Schafer C, Heiss A, Woltje M, Jahnen-Dechent W. Tissue distribution and |activity testing suggest a similar but not identical function of fetuin-B and fetuin-A. Biochem J. |2003 Nov 15; 376(Pt 1): 135-45. |USBio References
No references available