FRAP1 (S2481) (Serine/Threonine-protein Kinase mTOR, Mechanistic Target Of Rapamycin, FK506-binding Protein 12-rapamycin Complex-associated Protein 1, FKBP12-rapamycin Complex-associated Protein, Rapamycin Target Protein 1, RAPT1, Mammalian Target Of Rapamycin, mTOR, FRAP, FRAP2)

F4150-34F

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Accession #

NP_004949

Shipping Temp

Blue Ice

Storage Temp

-20°C

Rabbit Anti-FRAP1 (S2481) (Serine/Threonine-protein Kinase mTOR, Mechanistic Target Of Rapamycin, FK506-binding Protein 12-rapamycin Complex-associated Protein 1, FKBP12-rapamycin Complex-associated Protein, Rapamycin Target Protein 1, RAPT1, Mammalian Target Of Rapamycin, mTOR, FRAP, FRAP2)

FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.

Applications

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic non-phosphopeptide corresponding to amino acid residues surrounding S2481 of human FRAP1 (KLH).

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human FRAP1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References

1.Dowling,R.J., Cancer Res. 67(22), 10804-10812 (2007). 2.Bai,X., Science 318(5852), 977-980 (2007). 3.Zhou,J., Proc. Natl. Acad. Sci. USA 104(41), 16158-16163 (2007). 4.Radulovic,S., J BUON 12 SUPPL 1, S151-S162 (2007).

USBio References

No references available

Technical Data

F4150-34F

Clone Type

Host

Source

Isotype

Grade

Applications

Crossreactivity

Accession #

Shipping Temp

Storage Temp

Rabbit Anti-FRAP1 (S2481) (Serine/Threonine-protein Kinase mTOR, Mechanistic Target Of Rapamycin, FK506-binding Protein 12-rapamycin Complex-associated Protein 1, FKBP12-rapamycin Complex-associated Protein, Rapamycin Target Protein 1, RAPT1, Mammalian Target Of Rapamycin, mTOR, FRAP, FRAP2)

References

USBio References