Mouse Anti-Fibrin Degradation Product, D-Dimer
Human D-dimer (DD), a specific degradation product of cross-linked fibrin, is released into the bloodstream during clot breakdown. It is used as an indicator of blood clots, venous thromboses or pulmonary embolisms.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Sandwich ELISA: Plasma samples should be diluted with 10mM Tris-HCl buffer, pH 7.5, 1M sodium chloride, 0.1% Tween-20. To avoid non-specific binding, the final sodium chloride concentration in plasma samples should be ≥0.5M. Optimal dilutions to be determined by the researcher.
Matched Pair
Suitable for use as the capture or solid phase antibody. Capture: F4197-23 Matched to F4197-17 or F4197-18 as the detection or conjugate (label) antibody.
Hydridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Homogenized fibrin clot.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human D-dimer, a specific degradation product of cross-linked fibrin. Does not crossreact with D-monomer or fibrinogen.
