Goat Anti-Fibrinogen (Coagulation Factor I) (FITC)
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking.
Applications
Suitable for use in Immunoelectrophoresis, Radial Immunodiffusion (Ouchterlony) and FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Fluorescein/IgG protein molar ratio (F/P)
~1.7
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Immunogen
Fibrinogen isolated from fresh canine plasma after removing prothrombin.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by DEAE chromatography.
Specificity
Recognizes canine fibrinogen. In immunoelectrophoresis and radial immunodiffusion|(Ouchterlony) against normal canine plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. May also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. Does not crossreact with any other component of dog plasma.
