Mouse Anti-Fibroblast Growth Factor, Basic (FGFb)
bFGF (FGF-2) is a member of a family of seven peptides that are potent regulators of cell proliferation, differentiation and function. bFGF stimulates the proliferation of cells of mesodermal origin and many cells of endodermal, ectodermal and neuroectodermal origin. bFGF also induces neuron differentiation, survival and regeneration and stimulates astrocyte migration. bFGF may also be involved in the production of a variety of pathological conditions arising from excessive cell proliferation.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:10,000 ELISA: 1:8000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant protein corresponding to human basic FGF expressed in yeast cells.
Form
Supplied as a liquid in PBS, pH 7.4.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the recombinant human basic FGF. Does not crossreact with recombinant human acidic FGF.
