Rabbit Anti-Flag (DYKDDDDK) Epitope Tag (MaxLight 650)
DYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. In Western Blot of bacterial extracts does not cross-react with any endogenous proteins.
