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F4524-72G-ML650 Rabbit Anti-Flag (DYKDDDDK) Epitope Tag (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Polyclonal
Host
Rabbit
Conjugate
MaxLight™650
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
DYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. In Western Blot of bacterial extracts does not cross-react with any endogenous proteins.
References
1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6: 1204-1210. 2. Slootstra, J.W. et al. (1997) Identification of new tag sequences with differential and selective recognition properties for anti-FLAG monoclonal antibodies M1, M2 and M5. Mol. Divers. 2: 156-164.
USBio References
No references available
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