Rabbit Anti-Flag (DYKDDDDK) Epitope Tag (HRP)
DYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000-1:50,000 Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to Enterokinase cleavage site, DYKDDDDK, conjugated to KLH. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA., 0.01% gentamicin sulfate. Reconstitute with 100ul sterile ddH2O. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes DYKDDDDK when fused to either the amino or carboxyl terminus of target proteins. Shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence. Does not crossreact with any endogenous proteins.
