Chicken Anti-Flag (DYKDDDDK) Epitope Tag
DYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)
The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
Applications
Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:5000-1:10,000 Western Blot: 1:2000-1:10,000 Immunofluorescence (IC): 1:500 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1906-10: IgY, Chicken-HRP Rb x Ch I1906-12: IgY, Chicken-FITC Rb x Ch I1906-14: IgY, Chicken-AP Rb x Ch
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Flag (DYKDDDDK) Epitope Tag, conjugated to KLH.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Flag Epitope Tag.
