Mouse Anti-Fluorescein Isothiocyanate (FITC) (PE)
Fluorescein isothiocyanate (FITC) is a small fluoresent molecule that is commonly conjugated to antibodies for immunofluorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal amplification.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by DEAE chromatography from ascites.
Specificity
Preferential binding of isomer 6, reduced binding to isomer 5.
