Technical Data

F5385-01
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Accession #
NP_064450
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-FMN2 (Formin-2)

FMN2 is a protein belonging to the Formin homology family and Cappuccino subfamily (due to their high homology to the Drosophila 'cappuccino' protein). An FH1 domain with 11 proline repeats and a FH2 domain are conserved in both FMN1 and FMN2. It plays an important role in cytoskeletal organization and/or establishment of cell polarity. FMN2 may also be involved in maintenance of the meiotic spindle. Reports suggest that upon DNA damage, FMN2 gets phosphorylated by ATM or ATR. Northern blot analysis revealed the expression of FMN2 exclusively in both developing and mature central nervous system tissues. It is expressed in various human tumors, including parathyloid tumor, glioblastoma, retinoblastoma and chondrosarcoma.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.25-0.5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human brain lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa650-700 of human FMN2.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human FMN2. Species sequence homology: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Matsuoka, S. et al. Science. 316:1160-1166 (2007). 2. Leader, B. et al. Mech. Dev. 93:221-231 (2000). 3. Leader, B. et al. Nature Cell Biol. 4:921-928 (2002).
USBio References
No references available
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