Mouse Anti-Follicle Stimulating Hormone beta (FSHb) (HRP)
Follicle-Stimulating Hormone beta (FSH beta) is a 21kD component of the glycoprotein hormone FSH (Accession # NP_000501). It is secreted by the pituitary in noncovalent association with an alpha chain that is also a component of luteinizing hormone, thryoid stimulating hormone, and chorionic gonadotropin. FSH promotes ovarian follicle maturation and spermatogenesis through interactions with FSH R. Human FSH beta shares ~90% aa sequence identity with mouse and rat FSH beta.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 1:3000 Optimal dilutions to be determined by the researcher.
Affinity Constant
9x10e9 L/mole
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human Follicle Stimulating Hormone
Form
Supplied as a liquid in buffer, pH 7.6, thimerosal. Labeled with Horseradish Peroxidase (HRP).
Specificity
Recognizes human Follicle Stimulating Hormone beta (FSHb). Crossreactivity: bHCG, tHCG, LH and Prolactin, <1%
