Mouse Anti-Fragile X Mental Retardation Protein 1 (FMR1, FMR-1, FMRP, FRAXA, POF, POF1, Protein FMR-1)
Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Applications
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
ELISA: 1:500-5000 Western Blot: 1:500 Immunocytochemistry: 1:500-5000 Immunohistochemistry (Frozen and paraffin): 1:500-5000 Optimal dilutions to be determined by the researcher.
Positive Control
Includes negative control mouse IgG antibody and control primers specific for mouse Iqsec1.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein with a full length FMRP (human).
Form
Supplied as a liquid.
Specificity
Recognizes human FMRP. Species Crossreactivity: mouse and rat.
