Mouse Anti-FLI1 (Friend Leukemia Virus Integration 1 Transcription Factor, Transcription Factor ERGB, Proto-oncogene FLI-1)
FLI1 is a member of ETS family of transcription factors and is a proto-oncogene involved in erythroleukemia and Ewings sarcoma. FLI1 was originally identified as a common proviral insertion (Friend leukemia insertion site 1) and found in 75% of erythroleukemia cell clones induced by the Friend mouse leukemia virus. A chromosomal translocation between the FLI1 gene and EWS gene is found in most Ewing sarcomas. FLI1 has anti-apoptotic acivities and also interferes with nuclear hormone receptors.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
FLI1 (AAH01670, 1-453aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity
Recognizes human FLI1. Species Crossreactivity: rat.
