Rabbit Anti-Frizzled 5 (FZD5)
The Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis. The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), beta- and gamma- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors. Frizzled 5 is believed to function as the receptor for the Wnt5a ligand.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 7ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to extracellular domain of human FZD5 at N-terminal (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Frizzled-5.
