Technical Data

F7080
Clone Type
Polyclonal
Host
Sheep
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Sheep Anti-Fibromodulin (FMOD, FM, Collagen-binding 59kD Protein)

Fibromodulin (FMOD; also FM and collagen-binding 59kD protein) is a secreted, 60-70kD class II member of the SLRP family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-B. Fibromodulin is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers. Mature human Fibromodulin is 358aa in length (aa19-376). It contains 12 LRRs (Leu-rich repeats) (aa98-376), with the seventh and eleventh LRR principally responsible for collagen type I binding. Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation. There is one splice form that shows a deletion of aa88-101. Over aa76-376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1ug/ml Western Blot: 0.3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived recombinant human Fibromodulin (aa75-376).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Fibromodulin. Species Crossreactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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