Mouse Anti-FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF3A, TCF3A)
FOXA1 (HNF3A, Hepatocyte nuclear factor 3- alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor alpha, in each of the tumor type respectively (Lupien, 2008). In the case of ERa, FOXA1 has gained notice because of its interactions with the cis- regulatory regions in heterochromatin and because it enhances the interaction of ERa with chromatin (Badve, 2007).
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:2000 Immunofluorescence (IC):1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa7-86 at the N-terminal of human GST-FOXA1.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human FOXA1 (HNF3a) at ~49kD. Species Crossreactivity: mouse
