Mouse Anti-FXYD Domain-containing Ion Transport Regulator 1, phosphorylated (Ser68) (Phospholemman, FLM, Fxyd1, Plm)
PLM (FXYD1)is a member of a family of small membrane proteins that share a 35aa signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. PLM may be phosphorylated by several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic phosphopeptide mapping to a fragment of residues surrounding Ser68 human PLM, UniProt Accession #NP_062376; Q9Z239 |Cellular Localization: Membrane; Single-pass type 1 membrane protein
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse PLM when phosphorylated at Ser68 at ~10kD.
