Technical Data

F9500-03K
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
FYN-01
Grade
Affinity Purified
Applications
IC IHC IP WB
Crossreactivity
Hu Mo
Gene ID
2534
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Fyn (p59Fyn, SLK, SYN)

Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.

Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein fragment corresponding to aa7-176 from human FYN expressed bacterially.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human FYN. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71.
USBio References
No references available
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