Rabbit Anti-G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/G9a, Protein G9a ) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
G9a (BAT8 or Histone-lysine N-methyltransferase) is thought to be involved in intracellular protein-protein interaction and is a lysine-preferring histone methyltransferase. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. The presence of the ankyrin repeats also suggests that G9a is involved in intracellular protein-protein interaction. Like Suv39h1, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10- to 20-fold higher activity. G9a is localized to the nucleus but not in the repressive chromatin domains of centromeric loci, in which Suv39h1 family proteins were localized. This protein is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a also methylates histone H1.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated linear peptide corresponding to G9a (BAT8). Cellular Localization: Nucleus. Species sequence homology: rat and canine.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human G9a. Species Crossreactivity: mouse.
