Mouse Anti-β-Galactosidase (E. coli)
EC=3.2.1.23, b-gal
Beta galactosidase is a a metalloenzyme that that splits lactose into glucose and galactose and is coded by a gene (lac z) in the lac operon of Escherichia coli. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
beta-Galactosidase purified from E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes a non-catalytic center epitope of E. coli beta-galactosidase in all species.
