Rabbit Anti-Galanin Receptor 2 (GALR2)
Galanin is a 29 aa C-terminally amidated (30 aa, non-amidated in humans), highly conserved but unique neuroendocrine peptide originally isolated from intestine. The first 14 aa are fully conserved in almost all species. Galanin is found in the brain and the gut. It modulates a variety of physiological processed including cognition/memory, sensory/pain processing, neurotransmitter/hormone secretion, and feeding behavior. Several N-terminally elongated (-7-29 and -9-29) or truncated biologically active forms of galanin have also been isolated. Galanin antagonists are chimeric peptides generated by linking the amino terminal portion of galanin to substance P (galantide, M15), bradykinin (M35), the neurokinin antagonist spantide (C7) or an idealized alpha helical region (M40) (see review in refs 2 by Kask et al 1995).
Galanin mediated its biological effects by interacting with high affinity cell surface G-protein coupled receptors (GALR1-3). GALR2 (human 387 aa; chromosome 17q25.3; rat 372 aa) is ~40% homologous with rat GALR1. The rat and human GALR2 are ~87% conserved. GALR2 is widely distributed in various areas of the brain and several peripheral tissues. GALR2 binds galanin, N-terminal galanin fragments, and chimeric peptides. However, galanin 2-29 bind GALR2 with higher affinity than GALR1.
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well Western Blot: 1-10ug/ml using ECL technique. Optimal dilutions to be determined by the researcher.
Note: The appropriate control immunogenic peptide (G1043-10H) is available to confirm specificity of antibodies.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 16 aa peptide sequence within the cytoplasmic, C-terminus of mouse GLAR2 (1) was coupled to KLH
Form
Supplied as liquid in PBS, pH 7.4, containing 0.1% BSA and 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Mouse peptide sequence has high homology with rat (15/16 aa; 90%) and human (14/16 aa; 87%) GALR2. No significant homology is seen with GLAR1, GALR3 or any other G-protein coupled receptors. Antibody crossreactivity with GALR2 from various species is not known.
