Technical Data

G1044-50J
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
Gal397
Grade
Affinity Purified
Applications
IC IF WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Galectin-3 (Gal-3, 35kD Lectin, Carbohydrate-binding Protein 35, CBP 35, Galactose-specific Lectin 3, Galactoside-binding Protein, GALBP, IgE-binding Protein, L-31, Laminin-binding Protein, Lectin L-29, Mac-2 Antigen, LGALS3, MAC2)

Galectin-3 (galactose-specific lectin 3) is a 30-32kD glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and eBP. It is a member of the animal B-galactoside-binding lectin family that is expressed in the cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to B-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Structurally, Galectin-3 possesses NWGR motif that is conserved in the BH-1 domain of the BCL-2 family and functions as an apoptotic molecule.

Applications
Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): ≤0.5ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant protein corresponding to aa151-251 of human Galectin-3
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by affinity chromatography
Specificity
Recognizes human Galectin-3. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kim, H-R. C., et al. 1999. Cancer Res. 59:4148. 2. Reljic, R., et al. 2004. Immunol. Lett. 93:51. 3. Takenaka, Y. et al. 2004. Mol. and Cell. Biol. 24:4395. 4. Joo, GH., et al. 2001. J. Leukoc. Biol. 69:555.
USBio References
No references available
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