Goat Anti-GAPDHS (Glyceraldehyde-3-phosphate Dehydrogenase, Testis-specific, Spermatogenic Cell-specific Glyceraldehyde 3-phosphate Dehydrogenase 2, GAPDH-2, Spermatogenic Glyceraldehyde-3-phosphate Dehydrogenase, GAPD2, GAPDH2, GAPDS, HSD35, HSD-35)
GAPDS is a 40-44kD member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56kD in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408aa in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa223-226. One splice variant shows a 9aa insertion after Ser220. Over aa75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa75-222 from human GAPDHS expressed in E. coli.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GAPDHS. Species sequence homology: recombinant human GAPDH and recombinant mouse GAPDH (~1%).
