Technical Data

G2023-10
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu Mo
Accession #
NP_055106, O75600
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GCAT (Glycine Acetyltransferase, AKB Ligase, Aminoacetone Synthetase, 2-amino-3-ketobutyrate Coenzyme A Ligase Mitochondrial, KBL)
EC=2.3.1.29

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the Center region of human GCAT (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human GCAT. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Edgar,A.J., Polak,J.M.Eur. J. Biochem. 267(6), 1805-1812 (2000). 2.Tressel,T., Thompson,R., J. Biol. Chem. 261(35), 16428-16437 (1986).
USBio References
No references available
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