Technical Data

G2033-63
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_000812.2; NP_001135741.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-GGCX (Gamma-glutamyl Carboxylase, GC, FLJ26629, Vitamin K-dependent gamma-carboxylase, Vitamin K gamma Glutamyl Carboxylase, VKCFD1)
vitamin K-dependent gamma-carboxylase, VKCFD1, FLJ26629, gamma-glutamyl carboxylase, GGCX

This gene encodes an enzyme which catalyzes the posttranslational modification of vitamin K-dependent protein. Many of these vitamin K-dependent proteins are involved in coagulation so the function of the encoded enzyme is essential for hemostasis. Mutations in this gene are associated with vitamin K-dependent coagulation defect and PXE-like disorder with multiple coagulation factor deficiency. Multiple transcript variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:32,000 Western Blot: 0.3-1ug/ml detects ~85kD band observed in Human Muscle lysates (calculated MW of 85kD according to NP_000812.2). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-PPESNPDPVHSE, from the C-Terminus of human GGCX (NP_000812.2; NP_001135741.1).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human GGCX. Species sequence homology: canine. Expected to recognize both isoforms (NP_000812.2; NP_001135741.1).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Li Q, Grange DK, Armstrong NL, Whelan AJ, Hurley MY, Rishavy MA, Hallgren KW, Berkner KL, Schurgers LJ, Jiang Q, Uitto J, Mutations in the GGCX and ABCC6 genes in a family with pseudoxanthoma elasticum-like phenotypes. J Invest Dermatol. 2009 Mar;129(3):553-63. Epub 2008 Sep 18.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net