Guinea pig Anti-Glial Filament Protein (Not for Export EU)
The 56kD GFAP protein is the main subunit of intermediate filaments of glial cells and astrocytes.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:2000 Immunohistochemistry (Frozen): 1:100; Incubate for 1 hour at RT. Immunohistochemistry (Paraffin): 1:50; Incubate >1 hr at RT; extended time for paraffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Glial Filament Protein purified from bovine spinal cord.
Form
Supplied as a liquid, 0.5% BSA, 0.09% sodium azide.
Purity
Serum
Specificity
Recognizes the bovine Glial Filament Protein (Mr 52,000 polypeptide). Detects astrocytomas, gangliomas, meulloblastomas and certain teratomas. Species Crossreactivity: human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Gould VE, Koukoulis GK, Jansson DS, Nagle RB, Franke WW, Moll R: Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic, and neoplastic breast. Am J Pathol 137, 1143-1155 (1990).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970 Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net
