Technical Data

G2040-39J
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)
Rabbit Anti-Glucagon-like Peptide 2 Receptor (GLP2R)

Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis. The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration. GLP2, also a processed active peptide with 33aa, (chr 2q36-q37). GLP2 regulates gastric motility, gastric acid secretion, intestinal hexose transport, and increases the barrier function of the gut epithelium. It significantly enhances the surface area of the mucosal epithelium via stimulation of crypt cell proliferation. The actions of GLP2 are transduced by the GLP2 receptor, a G-protein-coupled receptor, activation of receptor signaling in heterologous cells promotes resistance to apoptotic injury in vitro, as such it may potentially be useful in treatment of injury and dysfunction of intestinal mucosal epithelium. GLP2 Receptor, a 550aa protein in rat and 553aa in human (chr 17p13.3).

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1-10ug/ml using Chemiluminescence technique. ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml (See G2040-39K).
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide, 18aa corresponding to N-Terminus, extracellular|of human GLP2R.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GLP2R.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Donald G. Munroe (1999 PNAS) 96:1569-1573 ;  2. Yves Rouille, JBC (1995) 270: 26488-96, 3. Daniel, J. Drucker, (2001) Journal of Clinical Endocrinology and Metabolism, 86(4):1759-1764.
USBio References
No references available
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