Goat Anti-GPI, Glucose-6-Phosphate Isomerase (Neuroleukin (aa81-93))
sperm antigen-36, phosphosaccharomutase;, phosphohexose isomerase, phosphohexomutase, phosphoglucose isomerase, oxoisomerase;, neuroleukin, hexosephosphate isomerase;, hexose monophosphate isomerase, glucose-6-phosphate isomerase, autocrine motility factor;, SA-36, PGI, NLK, GNPI, AMF, glucose phosphate isomerase, GPI
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:128,000 Western Blot: 0.1-0.3ug/ml ~60kD band observed in Human Heart lysates (calculated MW of 63.1kD according to NP_000166.2). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RERMFNGEKINYT, from the internal region (near N Terminus) of human GPI (NP_000166.2).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human GPI. Species sequence homology: porcine.
