Rabbit Anti-Glucose Regulated Protein 94 (Grp94, gp96, ECGP, Endoplasmin, Heat Shock Protein 90kD beta Member 1, HSP90b1, Stress Inducible Tumor Rejection Antigen gp96, Tumor Rejection Antigen 1, TRA1)
GRP94 is a 803aa protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-2ug/ml Immunohistochemistry (paraffin): 10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
3T3 and RAW cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa590-640 of human GRP94.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human GRP94. Species Crossreactivity: chimpanzee, equine, mouse, monkey. Species sequence homology: rat, bovine, zebrafish, xenopus, chicken.
